Molecular and Industrial Biotechnology group. GBMI

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Pere Garriga Contributions

Scientific contributions

Communications to meetings (selected)

Meetings organized
  • 2012 Member of the Scientific Committee of the XII Spanish Biophysical Society. Barcelona, 3-6 July.
  • 2012 Member of the Organizing Committee of the 100th Anniversary of the Catalan Biological Society. Barcelona, 9-12 July
  • 2009 Organizer of the Worshop of the Section of Biophysics of the Institute of Catalan Studies. 16 September.
  • 2008 13th International Conference on Retinal Proteins. Scientific secretary and co-organizer with Prof. Esteve Padrós. 15-19 June.
Communications
  • Reyes-Alcaraz A, Martinez-Archundia M, Garriga P. Inorganic salt effects on the stability of active and inactive conformation of the G-protein coupled receptor rhodopsin. XI Congreso de la Sociedad de Biofísica de España. Murcia, 1-4 de Junio del 2011.
  • Martinez M, Cordomi A, Garriga P, Perez JJ. Conformational Studies of the M3 Acetylcholine Muscarinic Receptor and its Binding Site. XI Congreso de la Sociedad de Biofísica de España. Murcia, 1-4 de Junio del 2011.
  • Ramon E, Toledo D, Reyes-Alcaraz A, Morillo M, Garriga P. Efficient G-protein activation by fully-functional rhodopsin in the dark by the release of critical structural constraints. XXXIV congreso de la Sociedad Española de Bioquímicay Biología Molecular. Barcelona, 5-8 de septiembre de 2011.
  • Ramon E, Toledo D, Aguilà M, Cordomi A, Mendes HF, Cheetham ME, Garriga P. Molecular mechanisms for retinal diseases caused by mutations at Gly90 in the visual G protein coupled receptor, rhodopsin. XXXIV congreso de la Sociedad Española de Bioquímica y Biología Molecular. Barcelona, 5-8 de septiembre de 2011.
  • Ramon E, Bosch-Presegué L, Toledo D, Cordomí A, Garriga P. Photointermediate Stability Changes in Rhodopsin Mutants Associated with Retinitis Pigmentosa. XI Congreso de la Sociedad de Biofísica de España. Murcia, 1-4 de Junio del 2011.
  • Tena M, Borroto-Escuela DO, Romero-Fernandez W, Franch de Armas O, Perez JJ, Fuxe K, Garriga P. XXXIV congreso de la Sociedad Española de Bioquímica y Biología Molecular. Barcelona, 5-8 de septiembre de 2011.
  • D. Toledo, M. Aguilà, and P. Garriga: “G90 mutants and the molecular mechanism of hereditary retinophaties” 13th International Conference on Retinal Proteins (Barcelona, 2008)
  • D. Toledo, A. Cordomí, M.G. Proietti, L.J. del Valle, J.J. Perez, P. Garriga, and F. Sepulcre: “The structural characterization of a zinc high-affinity binding site in rhodopsin” 13th International Conference on Retinal Proteins (Barcelona, 2008)
  • A. Reyes, and P. Garriga: “The salt effect on the thermal stability of rhodopsin revisited” 13th International Conference on Retinal Proteins (Barcelona, 2008)
  • M. Martinez, A. Cordomi, L. Iarriccio, P. Garriga, and J.J. Perez: “Molecular dynamic simulations of M3 muscarinic acetylcholine receptor” 13th International Conference on Retinal Proteins (Barcelona, 2008)
  • D. O. Borroto-Escuela, M. Perez-Alea, W. Romero, D. Toledo, and P. Garriga: “Inhibition of rhodopsin mutants cell death by chimeric rhodopsin-muscarinic receptor C-terminal tail receptors in cell culture” 13th International Conference on Retinal Proteins (Barcelona, 2008)
  • P. Garriga:“Rhodopsin mutants associated with retinal diseases: new insights into the structure and function”13th International Conference on Retinal Proteins. Invited talk.(Barcelona, 2008)
  • E. Ramon, E.Y. Zerniy, L. Bosch, A. Cordomí, J. Manyosa, J.J. Pérez, P.P. Philippov, I.I. Senin, P. Garriga: “ Role of the cytoplasmic region of helices 3 and 6 in rhodopsin conformational properties” 10TH VISION RESEARCH CONFERENCE Rhodopsin -Advances and Perspectives 28 -29 APRIL 2006, FORT LAUDERDALE, FL, USA
  • L. Bosch, M. Dominguez, D. Toledo, M. Morillo, R. Alvarez, A. R. de Lera, P. Garriga: “Effect of the chromophore C7-methyl substitution on rhodopsin regeneration” 10TH VISION RESEARCH CONFERENCE. Rhodopsin –Advances and Perspectives 28 -29 APRIL 2006, FORT LAUDERDALE, FL, USA
  • M. Torres, I. Roman, E. Ramon, P.P. Philippov, I.I. Senin, P. Garriga, J. Manyosa “Phosphorylation of cytoplasmic rhodopsin mutants associated with retinitis pigmentosa” 6th European Symposium of the Protein Society (Barcelona, 2005)
  • E. Ramon, L. Bosch, A. Cordomi, J. Manyosa, J.J. Perez, P.P. Philippov, I.I. Senin, P. Garriga “Role of the cytoplasmic region of helices 3 and 6 on the structure, stability and phosphorylation of the G-protein coupled receptor rhodopsin” 6th European Symposium of The Protein Society (Barcelona, 2005)
Peer reviewed publications (selected)
  • Tena-Campos M, Ramon E, Rivera D, Borroto-Escuela DO, Romero-Fernandez W, Fuxe K, and Garriga P. G-protein-Coupled Receptors Oligomerization: Emerging Signaling Units and New Opportunities for Drug Design. Current Peptide and Protein Science (2014), in the press
  • Srinivasan S, Ramon E, Cordomí A, and Garriga P. Binding Specificity of Retinal Analogs to Photoactivated Visual Pigments Suggest Mechanism for Fine-Tuning GPCR-Ligand Interactions, Chemistry & Biology (2014), http://dx.doi.org/10.1016/j.chembiol.2014.01.0063
  • Aguilà M, Bevilacqua D, McCulley C, Schwarz N, Athanasiou D, Kanuga N, Novoselov S, Lange CAK, Ali R, Bainbridge JW, Gias C, Coffey PJ, Garriga P, and Cheetham ME. Hsp90 inhibition protects against inherited retinal degeneration. Human Molecular Genetics (2013) Dec 16. [Epub ahead of print] doi: 10.1093/hmg/ddt613
  • Sánchez-Martín MJ, Ramon, E, Torrent-Burgues J and Garriga P. Improved Conformational Stability of the Visual G-protein Coupled Receptor Rhodopsin by Specific Interaction with Docosahexaenoic Acid Phospholipid. ChemBioChem. 14,639-644 (2013).
  • Fuxe K, Borroto-Escuela DO, Romero-Fernandez W, Diaz-Cabiale Z, Rivera A, Ferraro L, Tanganelli S, Tarakanov AO, Garriga P, Narváez JA, Ciruela F, Guescini M, Agnati LF. Extrasynaptic neurotransmission in the modulation of brain function. Focus on the striatal neuronal-glial networks. Front Physiol. (2012); 3:136. Epub 2012 Jun 4.
  • Fuxe K, Borroto-Escuela DO, Romero-Fernandez W, Tarakanov AO, Calvo F, Garriga P, Tena M, Narvaez M, Millón C, Parrado C, Ciruela F, Agnati LF, Narvaez JA, Díaz-Cabiale Z. On the existence and function of galanin receptor heteromers in the central nervous system. Front Endocrinol. 3:127. doi: 10.3389/fendo.2012.00127. Epub 2012 Oct 26. (2012).
  • Borroto-Escuela DO, Romero-Fernandez W, Garriga P, Ciruela F, Narvaez M, Tarakanov AO, Palkovits M, Agnati LF and Fuxe K. G-protein coupled receptor heterodimerization in the brain. Methods Enzymol.521, 281-294. (2013).
  • Sánchez-Martín MJ, Ramon E and Garriga P. Fluorescence studies of DHA liposomes ability to stabilize the G-protein-coupled receptor rhodopsin. Luminiscence (in press) DOI 10.1002/bio.2432.
  • Martinez-Archundia M, Cordomi A, Garriga P., Perez JJ. Molecular modeling of the M3 acetylcholine muscarinic receptor and its binding site. J Biomed Biotechnol. 2012;2012:789741. doi: 10.1155/2012/789741. Epub 2012 Feb 277.
  • Borroto-Escuela DO, Romero-Fernandez W, García-Negredo G, Correia PA, Garriga P, Fuxe K, Ciruela F. Dissecting the Conserved NPxxY Motif of the M3 Muscarinic Acetylcholine Receptor: Critical Role of Asp-7.49 for Receptor Signaling and Multiprotein Complex Formation. Cellular Physiology and Biochemistry (2011, inthe press).
  • Reyes-Alcaraz A, Martínez-Archundia M, Ramon E, Garriga P. Salt effects on the conformational stability of the G-protein-coupled receptor rhodopsin. Biophysical J. 101, 2798-2806 (2011).
  • Toledo D, Ramon E, Aguilà M, Cordomí A, Pérez JJ, Mendes HF, Cheetham ME, Garriga P. Molecular mechanisms of disease for mutations at gly-90 in rhodopsin. J Biol Chem. 286, 39993-40001 (2011).
  • Romero-Fernandez W, Borroto-Escuela DO, Perez Alea M, Garcia-Mesa Y, Garriga P. Altered trafficking and unfolded protein response induction as a result of M3 muscarinic receptor impaired N-glycosylation. Glycobiology. 21, 1663-1672(2011).
  • Alea MP, Borroto-Escuela DO, RomeroRomero-Fernandez W, Fuxe K, Garriga P. Differential expression of muscarinic acetylcholine receptor subtypes in Jurkat cells and their signaling.J Neuroimmunol. 237, 13-22 (2011)
  • Romero-Fernandez W, Garriga P, Borroto-Escuela DO. Overproduction of human M₃ muscarinic acetylcholine receptor: an approach toward structural studies. Biotechnol Prog. 27, 838-845 (2011)
  • Bosch-Presegué L, Ramon E, Toledo D, Cordomí A, Garriga P. Alterations in the photoactivation pathway of rhodopsin mutants associated with retinitis pigmentosa. FEBS J. 278, 1493-1505 (2011).
  • Borroto-Escuela DO, Correia PA, Romero-Fernandez W, Narvaez M, Fuxe K, Ciruela F, Garriga P. Muscarinic receptor family interacting proteins: role in receptor function. J Neurosci Methods. 195,:161-169 (2011).
  • Bosch-Presegué L, Iarriccio L, Aguilà M, Toledo D, Ramon E, Cordomí A, Garriga P. Hydrophobic amino acids at the cytoplasmic ends of helices 3 and 6 of rhodopsin conjointly modulate transducin activation. Arch Biochem Biophys. 506, 142-149 (2011). 1
  • Komolov KE, Aguilà M, Toledo D, Manyosa J, Garriga P, Koch KW. On-chip photoactivation of heterologously expressed rhodopsin allows kinetic analysis of G-protein signaling by surface plasmon resonance spectroscopy. Anal Bioanal Chem. 397, 2967-2976 (2010)
  • Borroto-Escuela DO, García-Negredo G, Garriga P, Fuxe K, Ciruela F. The M(5) muscarinic acetylcholine receptor third intracellular loop regulates receptor function and oligomerization. Biochim Biophys Acta. 1803, 813-825 (2010).
  • Borroto-Escuela DO, Correia PA, Perez Alea M, Narvaez M, Garriga P, Fuxe K, Ciruela F. Impaired M(3) muscarinic acetylcholine receptor signal transduction through blockade of binding of multiple proteins to its third intracellular loop. Cell Physiol Biochem. 25, 397-408 (2010)
  • Kosmaoglou, M., Kanuga, N., Aguilà, M., Garriga, P., Cheetham, M.E. A dual role for EDEM1 in the processing of rod opsin. J Cell Sci. 122, 4465-4472 (2009).
  • D. Toledo, A. Cordomi, M.G. Proietti, M. Benfatto, L. J. del Valle, J. J. Perez, P. Garriga and F. Sepulcre Structural characterization of a zinc high-affinity binding site in rhodopsin Photochemistry and Photobiology 85, 479-484 (2009)
  • M.Aguila, D. Toledo, M. Morillo, M. Dominguez, R. Alvarez, A. R. de Lera, and P. Garriga. Structural coupling of 11-cis-7-methylretinal and amino acids at the retinal binding pocket of rhodopsin Photochemistry and Photobiology 85, 485-493 (2009)
  • A. Reyes, T. Tzanov and P. Garriga: Stabilization of membrane proteins: the case of G-protein-coupled receptors. Engineering in Life Sciences 8, 207-217 (2008)
  • A. Cordomí, E.Ramon, P. Garriga, J.J. Pérez: Conformational rearrangements at the cytoplasmic side of helices 3 and 6 of rhodopsin from molecular dynamics simulations of point mutants. Journal of Biomolecular Structure and Dynamics 25, 573-587 (2008)
  • E. Ramon, A. Cordomí, L. Bosch, E.Y. Zernii, I.I. Senin, J. Manyosa, P.P.Philippov, J.J. Perez, P. Garriga: Critical role of electrostatic interactions of amino acids at the cytoplasmic region of helices 3 and 6 in rhodopsin conformational properties and activation. Journal of Biological Chemistry 282, 14272-14282 (2007)
  • L. Bosch, A. Cordomí, M. Domínguez, D. Toledo, M. Morillo, R. Álvarez, J.J. Pérez, A.R. de Lera, P. Garriga: A methyl group at C7 of 11-cis-retinal allows chromophore formation but affects rhodopsin activation. Vision Research 46, 4472- 4481 (2006)
  • I.I.Senin, L. Bosch, E. Ramon, E.Y. Zernii, J. Manyosa, P.P.Philippov, P. Garriga: Ca2+/recoverin dependent regulation of phosphorylation of the rhodopsin mutant R135L associated with retinitis pigmentosa. Biochemical and Biophysical Research Communications 349, 346-352 (2006)
  • J-M. Kim, J. Hwa, P. Garriga, P.J. Reeves, U.L RajBhandary, H.G. Khorana: Light-driven Activation of β2-Adrenergic Receptor Signaling by a Chimeric Rhodopsin Containing the β2-Adrenergic Receptor Cytoplasmic Loops. Biochemistry 44, 2284¬2292 (2005)
  • L. Bosch, L. Iarriccio and P. Garriga: New prospects for drug discovery from structural studies of rhodopsin. Current Pharmaceutical Design 11, 2243-2256 (2005).